pet28a backbone (Addgene inc)
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pet28a backbone
Pet28a Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a+backbone/pET-28a(%2B)-FGF2-K128N+(Plasmid+%23120284)/pm41690922-369-11-13
Average 94 stars, based on 11 article reviews
Pet28a Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a+backbone/pET-28a(%2B)-FGF2-K128N+(Plasmid+%23120284)/pm41690922-369-11-13
Average 94 stars, based on 11 article reviews
pet28a backbone - by Bioz Stars,
2026-09
94/100 stars
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Polymerase Chain Reaction:Article Title: In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates Article Snippet: .. The 6xHis-MBP tag was PCR-amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Article Title: Unstructured mRNAs form multivalent RNA-RNA interactions to generate TIS granule networks Article Snippet: .. The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Construct:Article Title: In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates Article Snippet: .. The 6xHis-MBP tag was PCR-amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Article Title: Unstructured mRNAs form multivalent RNA-RNA interactions to generate TIS granule networks Article Snippet: .. The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Clone Assay:Article Title: In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates Article Snippet: .. The 6xHis-MBP tag was PCR-amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Article Title: Unstructured mRNAs form multivalent RNA-RNA interactions to generate TIS granule networks Article Snippet: .. The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes. Article Snippet: .. Each protein was cloned into the multiple cloning site of the Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes Article Snippet: .. Each protein was cloned into the multiple cloning site of the Strep-tag:Article Title: In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates Article Snippet: .. The 6xHis-MBP tag was PCR-amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Article Title: Unstructured mRNAs form multivalent RNA-RNA interactions to generate TIS granule networks Article Snippet: .. The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Sequencing:Article Title: In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates Article Snippet: .. The 6xHis-MBP tag was PCR-amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Article Title: Unstructured mRNAs form multivalent RNA-RNA interactions to generate TIS granule networks Article Snippet: .. The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Article Title: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins Article Snippet: Human AZI1 (UniProt O14977) was cloned as pET28a-His 6 -AZI-Ctag (GenBank MW364946) to give the following organization: (M)GSS-linker, His 6 –tag, SSG-linker, AZI1, GSG-linker, C- tag. pET28a-TGFα-GSY-SPM (GenBank MW364948, Addgene plasmid #163615) includes mature TGFα sequence that was taken from residues 40–89 of human protransforming growth factor alpha (UniProt P01135). pET28a-TGFα-GSY-SPM has the following organization: (M), TGFα, GSY-linker, SPM, GSS-linker, His 6 -tag, SSG-linker, C-tag. .. DNA primers and gene fragments codon-optimized for E. coli expression were ordered from Integrated DNA Technologies before cloning into the Article Title: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins. Article Snippet: Human AZI1 (UniProt O14977) was cloned as pET28a-His6-AZI-Ctag (GenBank MW364946) to give the following organization: (M)GSS-linker, His6–tag, SSG-linker, AZI1, GSG-linker, Ctag. pET28a-TGFα-GSY-SPM (GenBank MW364948, Addgene plasmid #163615) includes mature TGFα sequence that was taken from residues 40–89 of human protransforming growth factor alpha (UniProt P01135). pET28a-TGFα-GSY-SPM has the following organization: (M), TGFα, GSY-linker, SPM, GSS-linker, His6-tag, SSG-linker, C-tag. .. DNA primers and gene fragments codon-optimized for E. coli expression were ordered from Integrated DNA Technologies before cloning into the Amplification:Article Title: Unstructured mRNAs form multivalent RNA-RNA interactions to generate TIS granule networks Article Snippet: .. The 6xHis-MBP tag was PCR amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into Cloning:Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes. Article Snippet: .. Each protein was cloned into the multiple cloning site of the Article Title: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins Article Snippet: Human AZI1 (UniProt O14977) was cloned as pET28a-His 6 -AZI-Ctag (GenBank MW364946) to give the following organization: (M)GSS-linker, His 6 –tag, SSG-linker, AZI1, GSG-linker, C- tag. pET28a-TGFα-GSY-SPM (GenBank MW364948, Addgene plasmid #163615) includes mature TGFα sequence that was taken from residues 40–89 of human protransforming growth factor alpha (UniProt P01135). pET28a-TGFα-GSY-SPM has the following organization: (M), TGFα, GSY-linker, SPM, GSS-linker, His 6 -tag, SSG-linker, C-tag. .. DNA primers and gene fragments codon-optimized for E. coli expression were ordered from Integrated DNA Technologies before cloning into the Article Title: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins. Article Snippet: Human AZI1 (UniProt O14977) was cloned as pET28a-His6-AZI-Ctag (GenBank MW364946) to give the following organization: (M)GSS-linker, His6–tag, SSG-linker, AZI1, GSG-linker, Ctag. pET28a-TGFα-GSY-SPM (GenBank MW364948, Addgene plasmid #163615) includes mature TGFα sequence that was taken from residues 40–89 of human protransforming growth factor alpha (UniProt P01135). pET28a-TGFα-GSY-SPM has the following organization: (M), TGFα, GSY-linker, SPM, GSS-linker, His6-tag, SSG-linker, C-tag. .. DNA primers and gene fragments codon-optimized for E. coli expression were ordered from Integrated DNA Technologies before cloning into the Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes Article Snippet: .. Each protein was cloned into the multiple cloning site of the Plasmid Preparation:Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes. Article Snippet: .. Each protein was cloned into the multiple cloning site of the Article Title: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins Article Snippet: Human AZI1 (UniProt O14977) was cloned as pET28a-His 6 -AZI-Ctag (GenBank MW364946) to give the following organization: (M)GSS-linker, His 6 –tag, SSG-linker, AZI1, GSG-linker, C- tag. pET28a-TGFα-GSY-SPM (GenBank MW364948, Addgene plasmid #163615) includes mature TGFα sequence that was taken from residues 40–89 of human protransforming growth factor alpha (UniProt P01135). pET28a-TGFα-GSY-SPM has the following organization: (M), TGFα, GSY-linker, SPM, GSS-linker, His 6 -tag, SSG-linker, C-tag. .. DNA primers and gene fragments codon-optimized for E. coli expression were ordered from Integrated DNA Technologies before cloning into the Article Title: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins. Article Snippet: Human AZI1 (UniProt O14977) was cloned as pET28a-His6-AZI-Ctag (GenBank MW364946) to give the following organization: (M)GSS-linker, His6–tag, SSG-linker, AZI1, GSG-linker, Ctag. pET28a-TGFα-GSY-SPM (GenBank MW364948, Addgene plasmid #163615) includes mature TGFα sequence that was taken from residues 40–89 of human protransforming growth factor alpha (UniProt P01135). pET28a-TGFα-GSY-SPM has the following organization: (M), TGFα, GSY-linker, SPM, GSS-linker, His6-tag, SSG-linker, C-tag. .. DNA primers and gene fragments codon-optimized for E. coli expression were ordered from Integrated DNA Technologies before cloning into the Article Title: BamA self-insertion drives inner-to-outer membrane remodelling in diderm Firmicutes Article Snippet: .. Each protein was cloned into the multiple cloning site of the Expressing:Article Title: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins Article Snippet: Human AZI1 (UniProt O14977) was cloned as pET28a-His 6 -AZI-Ctag (GenBank MW364946) to give the following organization: (M)GSS-linker, His 6 –tag, SSG-linker, AZI1, GSG-linker, C- tag. pET28a-TGFα-GSY-SPM (GenBank MW364948, Addgene plasmid #163615) includes mature TGFα sequence that was taken from residues 40–89 of human protransforming growth factor alpha (UniProt P01135). pET28a-TGFα-GSY-SPM has the following organization: (M), TGFα, GSY-linker, SPM, GSS-linker, His 6 -tag, SSG-linker, C-tag. .. DNA primers and gene fragments codon-optimized for E. coli expression were ordered from Integrated DNA Technologies before cloning into the Article Title: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins. Article Snippet: Human AZI1 (UniProt O14977) was cloned as pET28a-His6-AZI-Ctag (GenBank MW364946) to give the following organization: (M)GSS-linker, His6–tag, SSG-linker, AZI1, GSG-linker, Ctag. pET28a-TGFα-GSY-SPM (GenBank MW364948, Addgene plasmid #163615) includes mature TGFα sequence that was taken from residues 40–89 of human protransforming growth factor alpha (UniProt P01135). pET28a-TGFα-GSY-SPM has the following organization: (M), TGFα, GSY-linker, SPM, GSS-linker, His6-tag, SSG-linker, C-tag. .. DNA primers and gene fragments codon-optimized for E. coli expression were ordered from Integrated DNA Technologies before cloning into the other:Article Title: DNA-directed termination of RNA polymerase II transcription. Article Snippet: pZH81: Rat1 was PCR amplified using oligos oZH435 and oZH436 from yeast genomic DNA and ligated into |